<div style="text-align: justify;">Sub-clinical Mastitis is one of the major problems in India which is among the largest milk producing countries in the world, hampering national economy. Among 422 bovine milk samples screened from different cattle farms of West Bengal with history of infection and drop in milk yield by on-spot bromothymol blue (BTB) test, 371 samples were considered for collection and secondary screening by somatic cell count study. The collected milk samples showed significantly high average SCC value of 3.26-4.88 lakh cells/ml of milk which was indicative of infection. Samples (23, 6.53%) were found to be positive for Pseudomonas sp. showing distinct bluish-green pigmentation on cetrimide agar, typical results on morphological and in biochemical characterization. Presence of 19 (5.39%) Pseudomonas aeruginosa isolates were confirmed by fluorescent technique for detection of characteristic blue-green fluorescence by the pigment pyoverdin. PCR of these positive isolates revealed that 11 isolates were positive for toxA and 6 isolates for exoS genes with 2 isolates possessing both the genes. In Mice pathogenicity test, highest fatality (100%) was shown by the isolates with both the genes followed by isolates with toxA (66.67%) and exoS (41.67%) respectively with successful re-isolation of P. aeruginosa from the dead mice. Post-mortem examination of the dead mice showed marked haemorrhages on liver, blackish discoloration of intestines and haemorrhages on lungs. Antibiogram revealed that the isolates were highly sensitive to drugs like amikacin (89.47%), imipenem (84.21%), meropenem (78.95%) and cefoperazone-sulbactam (84.21%) but resistant to tetracycline (68.42%), piperacillin (63.16%), oxacillin (52.63%), mecilinam (47.37%) and others.</div>

<div style="text-align: justify;">Mice pathology study of toxA and exoS genes bearing <em>Pseudomonas aeruginosa </em>isolated from bovine Sub-clinical mastitis in West Bengal with their Antibiogram</div>

<div><strong>Background:</strong> <em>Pasteurella multocida</em> is an important bacterial pathogen that causes many major diseases of which haemorrhagic septiciemia (HS) in cattle and buffalo is responsible for catastrophic epizootics in India and South Asia. In India, the disease haemorrhagic septiciemia is considered as the most dreaded bacterial disease. Various host- and pathogen- specific determinants are responsible for disease outcome. Various bacterial virulence genes (<em>tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA</em>) have been proposed to play a key role in this interaction.<br />
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<strong>Methods:</strong> The present study was done to compare the gene and deduced amino acid sequence of transferrin binding protein gene (<em>tbpA</em>) gene of field isolates and vaccine strain of <em>P. multocida</em> B: 2.<br />
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<strong>Result:</strong> It was observed that <em>tbpA</em> gene of field and vaccine strains have similar nucleotide sequence except at positions 574 and 620. The sequence of <em>tbpA</em> gene was used for prediction of matured TbpA protein characteristics. The deduced amino acid sequences of 242 amino acids revealed 99% homology with TbpA of <em>P. multocida</em> and with a variety of other TonB-dependent receptor proteins, indicating that it belongs to the family of outer membrane receptors. Deduced amino acid sequence was found to be similar in field and vaccine strains except at 207<sup>th</sup> amino acid. In field isolates Leucine was there while in vaccine strain Phenyl alanine was found. These both amino acids are hydrophobic in nature so no change in physico-chemical property of TbpA is expected. From this study it is concluded that single amino acid difference between field isolate and vaccine strain might not cause change in its binding and physico-chemical property.</div>


Mice pathology study of toxA and exoS genes bearing Pseudomonas aeruginosa isolated from bovine Sub-clinical mastitis in West Bengal with their Antibiogram

Cloning and Sequence Analysis of Transferrin Binding Protein Gene A of Field and Vaccine Strains of Pasteurella multocida B:2

Mice pathology study of toxA and exoS genes bearing Pseudomonas aeruginosa isolated from bovine Sub-clinical mastitis in West Bengal with their Antibiogram

​Cloning and Sequence Analysis of Transferrin Binding Protein Gene A of Field and Vaccine Strains of Pasteurella multocida B:2

Cloning and Sequence Analysis of Transferrin Binding Protein Gene A of Field and Vaccine Strains of Pasteurella multocida B:2